C2410-09A

CD55 (DAF, Decay Accelerating Factor)

Size

£479.00

SKU:: C2410-09A
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human monocytes
Clone:: 7H134
Format:: Supplied as a liquid in PBS, pH 7.2, 0.5mg/ml gentamicin sulfate.
Extra Details:: Human CD55 is a GPI anchored protein that can protect cells from complement mediated lysis. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen): acetone fixation. Flow Cytometry: 10ug/ml labels 5x10e5 HPB-MLT cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 HPB-MLT human tumor cells were washed and incubated 45 minutes on ice with 80ul of C2410-09A at 10ug/ml. 2. Cells were washed twice and incubated with a goat x mouse 3. Cells were washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 1.44 log10 fluorescent units when compared to a Mouse IgG1 negative control (I1904-78R) at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.