C2410-02T
CD55 (DAF, Decay Accelerating Factor)
Size
£837.00
- SKU:
- C2410-02T
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Met1-Pro359 from mouse CD55 expressed in NSO cells.
- Clone:
- 11D35
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- CD55 (Decay-accelarating factor/DAF) is a glycoprotein member of the RCA family of molecules. It is found on blood cells, epithelium and endothelium, and serves both as a receptor for CD97, and a negative regulator of the C3 convertases, C4b2a and C3bBb. Mature mouse CD55 is the product of two genes that arose by duplication. There is a 55-60kD, 356aa, GPI-linked form that is ubiquitously expressed. This molecule contains four SUSHI domains (aa35-285), a Ser/Thr-rich region aa288-362, and a GPI-anchor at Gly362. There is also a 50kD, 379aa, type I transmembrane form that is testis-associated. It shows the same domain architecture and is 93% aa identical to the GPI-form. At least four GPI gene isoforms exist. They diverge after Ile285 and show deletions and substitutions. Over (aa35-359), mouse CD55 is 66% and 50% aa identical to rat and human CD55, respectively. Applications: Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug labels 10e6 cells using mouse splenocytes Direct ELISA: 0.5-1ug/ml; The detection limit for recombinant mouse CD55 is ~2ng/well. Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice