C2410-02A

CD55 (DAF, Decay Accelerating Factor)

Size

£691.00

SKU:: C2410-02A
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human fibroblast cell line.
Clone:: 3H1564 (BRIC 216)
Format:: Supplied as a liquid in TBS, 0.09% sodium azide.
Extra Details:: CD55 is a 70kD glycoprotein also known as Decay Accelerating Factor (DAF). Distributed on erythrocytes and other circulating blood cells and also on cells in non-haemopoietic tissue particularly epithelium and endothelium. Found specifically at the foetal-maternal interfaces in placenta.CD55 has reduced expression on individuals with paroxysmal nocturnal haemoglobinuria. This antibody has a functional binding affinity to erythrocytes of 8.7x10e7 M-1. The antigen is pronase and trypsin resistant and chymotrypsin sensitive. Applications: Suitable for use in Flow Cytometry, Western Blot and Functional assays. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells in 100ul Functional assays: Removal of sodium azide is recommended. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.