C2409-11M
CD54 (D1) (Intercellular Adhesion Molecule 1, ICAM-1, Major Group Rhinovirus Receptor, ICAM1)
Size
£479.00
- SKU:
- C2409-11M
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human monocytes
- Clone:
- 15.2
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
- Extra Details:
- Human CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes is weak but is upregulated on activated T and B lymphocytes and on monocytes. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: Capture Immunohistochemistry: Fixed cells and tissue Flow Cytometry: 1ug/ml Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x10e5 cultured Raji cells were incubated 45 min on ice with 80ul of C2409-11M at 1ug/ml. 2. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. 3.Cells stained positive with a mean shift of 1.58 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice