C2409-10H
CD54 (Intercellular Adhesion Molecule, ICAM-1) (PE)
Size
£704.00
- SKU:
- C2409-10H
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human endothelial cells
- Clone:
- 7H137 (8.4A6)
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- Human CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes is weak but is upregulated on activated T and B lymphocytes and monocytes. Applications: Suitable for use in FLISA and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (80ul/test) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured Raji cells were washed and incubated 45 minutes on ice with 80ul of C2409-10H at a 1:50 dilution. 2. Cells were washed three times, fixed and analyzed using a BD FACstar plus. 3. Cells stained positive with a mean shift of 2.47 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. 4. Binding was 91% blocked when pre incubated for 10 minutes with 20ul of 0.5mg/ml C2409-10E. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice