C2408-21A

CD53

Size

£479.00

SKU:: C2408-21A
Application:: Flow Cytometry
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human sezary cells
Clone:: 7H133
Format:: Supplied as a liquid in PBS, pH 7.5, 0.5mg/ml gentamicin sulfate.
Extra Details:: Human CD53 is a tetraspan cell surface glycoprotein found on all hemopoetic cells except platelets and red blood cells. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 5ug/ml Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x10e5 cultures Jurkat cells/tube were incubated 45 minutes on ice with 80ul of C2408-21A at 5ug/ml. 2. Cells were washed twice and incubated with IgG (FITC) goat anti-mouse, after which they were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.03 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.