C2405-12F
CD51 (DKFZp686A08142, Integrin alpha-V, MSK8, Vitronectin receptor subunit alpha, VNRA)
Size
£479.00
- SKU:
- C2405-12F
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human ocular melanoma cell line (V+B2)
- Clone:
- P2W7R
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
- Extra Details:
- Human CD51 is an integrin alpha v chain which can associate with five different integrin beta chains to form various adhesion molecules. The alpha v beta 3 integrin complex binds vitronectin at the RGD sequence and also can bind to fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Recognizes the alpha v molecule of 160kD. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured UM-SCC (squamous cell carcinoma) per tube were harvested by trypsinization, washed and pre-incubated 5 min with 20ul of 250ug/ml human IgG (to block nonspecific binding), after which they were incubated 45 min on ice with 80ul of anti-CD51 antibody at 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.32 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice