C2405-04A-FITC
CD49f (Laminin Receptor, Integrin alpha6, VLA alpha6, Platelet gpIc) (FITC)
Size
£837.00
- SKU:
- C2405-04A-FITC
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- a6b4 integrin purified from A431 cells
- Clone:
- 450-30A
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- CD49f is expressed by platelets, weakly by monocytes and by a subset of lymphocytes. CD49f is also widely expressed on epithelial tissues. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunohistochemistry: frozen Flow Cytometry: 10ul labels 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher. Recommended Positive Control Tissue (IHC): Tonsil Recommended Negative Controls: Mouse IgG1 Negative Control Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG:HRP; F(ab')2 rabbit anti mouse IgG:RPE conjugate; F(ab’)2 rabbit anti-mouse IgG:FITC Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice