C2405-02L1
CD49e (CD49 Antigen-like Family Member E, Fibronectin Receptor alpha, FNRA, Integrin alpha-F, Integrin alpha 5, Integrin alpha-5, ITGA5, Very Late Activation Protein 5 alpha, VLA-5, VLA5A)
Size
£825.00
- SKU:
- C2405-02L1
- Application:
- WB
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Phe42-Tyr995 from human CD49e, expressed in CHO cells.
- Clone:
- 11n34
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Integrin a5, is a 140-150kD integrin alpha chain that forms dimers with at least five beta chains including b1, 3, 5, 6, and 8. It is a 1018aa residue type I membrane protein with a large 962aa extracellular domain (ECD) and a short 32aa cytoplasmic tail. The N-terminal region of Integrin a5, which is important for ligand binding, contains seven FG-GAP (phenylalanyl-glycyl and glycyl-alanyl-prolyl) consensus repeats that fold into a b-propeller domain. Furin cleavage of the Integrin a5 ECD occurs after Gly 889, generating a disulfide-linked, heteromeric subunit Integrin a5 chain. Integrin a5-containing integrins bind multiple ECM molecules, including Vitronectin, Osteopontin, MMP-2, and TSP. The ECD of human Integrin 5 shares 92aa sequence identity with mouse Integrin a5 ECD. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml using K562 human chronic myelogenous leukemia cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice