C2404-65D
CD49d (Integrin alpha4, VLA4, LHR, Lymphocyte Homing Receptor) (PE)
Size
£448.00
- SKU:
- C2404-65D
- Purity:
- Purified
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Clone:
- PS/2
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide and a stabilizing agent. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- VLA-4 belongs to the beta1-integrin family, which functions both as cell-matrix and cell-cell receptors (1-3). As a matrix receptor VLA-4 binds to an alternatively spliced domain of fibronectin, while as a cellular receptor it associates with its counter-receptor ligand VCAM-1 (1,2). In addition to CD44/pgp-1, VLA-4 has been shown to be important for lymphopoiesis, and is also believed to be important for extravasation of lymphocytes into sites of inflammation as a second signal in addition to LFA/ICAM-1 (2,3). CD49d/VLA-4 is expressed in low amounts on resting lymphocytes and monocytes, is absent or minimally detectable on stromal cells, whereas virtually all lymphoid and myeloid cells in bone marrow and in long-term culture are positive (1,2). Applications: • Identification and enumeration of CD49d+ cells by flow cytometry 1, 2 • Studies of cell-cell interaction (1, 2) and tumor cell metastasis and adhesion 4 • Immunohistochemical studies 3 • Inhibition of lymphopoiesis in Whitlock-Witte long-term bone marrow cultures 1 • Retardation of myelopoiesis in Dexter-type cultures 1 • Immunoprecipitation 1 Recommended Dilution: Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice