C2404-47M
CD49d (VLA4a Chain, Very Late Antigen 4) (FITC)
Size
£620.00
- SKU:
- C2404-47M
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- HOON pre-B leukemia cell line.
- Clone:
- 3H1499 (44H6)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- The integrin alpha4 subunit is associates with either integrin beta1 (to form VLA-4) or beta7. The alpha4 integrin is a 150kD molecule which may be cleaved to 80kD and 70kD fragments. Expressed by lymphocytes, monocytes, eosinophils and thymocytes. VLA-4 mediates cell adhesion to VCAM-1 and fibronectin. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: Neat. Use 10ul to label 100ul whole blood or 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher. Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the SP2.0-Ag14 mouse myeloma cell line. Recommended Negative Controls: Mouse IgG1 Negative Control: FITC Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice