C2404-46G2

CD49d (Antigen CD49d, CD49d Antigen, CDw49d, Alpha 4 Subunit of VLA-4 Receptor, Integrin alpha IV, Integrin alpha 4, IA4, ITGA4, LPAM23, MGC90518, Very Late Activation Protein 4 Receptor Alpha 4 Subunit, VLA4, VLA-4)

Size

£550.00

SKU:: C2404-46G2
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: HOON pre-B leukemia cell line
Clone:: 3H1499 (44H6)
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: CD49d (VLA-4) inhibits adhesion of rat lymphocytes to cytokine-activated endothelial cells. It blocks binding of rat lymphocytes to purified VCAM-1 and to the CS-1 peptide of fibronectin. VLA-4 is a leukocyte integrin which plays an important role in lymphocyte adhesion and activation. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:40; 10ul labels 10e6 cells in 100ul Immunohistochemistry (Frozen): 1:50-1:100. Overnight incubation with C2404-46G2 and acetone fixation for frozen sections is recommended. Not suitable for use with paraffin sections. Optimal dilutions to be determined by the researcher. Hybridoma: SP2.0-Ag14 myeloma cells with spleen cells from Balb/c mice Positive Control for Immunohistochemistry: Human tonsil Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.