C2404-46G2-ML490
CD49d (Antigen CD49d, CD49d Antigen, CDw49d, Alpha 4 Subunit of VLA-4 Receptor, Integrin alpha IV, Integrin alpha 4, IA4, ITGA4, LPAM23, MGC90518, Very Late Activation Protein 4 Receptor Alpha 4 Subunit, VLA4, VLA-4) (MaxLight 490)
Size
£837.00
- SKU:
- C2404-46G2-ML490
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- HOON pre-B leukemia cell line
- Clone:
- 3H1499 (44H6)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. CD49d (VLA-4) inhibits adhesion of rat lymphocytes to cytokine-activated endothelial cells. It blocks binding of rat lymphocytes to purified VCAM-1 and to the CS-1 peptide of fibronectin. VLA-4 is a leukocyte integrin which plays an important role in lymphocyte adhesion and activation. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells in 100ul Immunohistochemistry (Frozen): Overnight incubation with C2404-46G2 and acetone fixation for frozen sections is recommended. Not suitable for use with paraffin sections. Optimal dilutions to be determined by the researcher. Hybridoma: SP2.0-Ag14 myeloma cells with spleen cells from Balb/c mice Positive Control for Immunohistochemistry: Human tonsil Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice