C2404-08D
CD49c (Integrin alpha3, Very Late Antigen3a chain, VLA3a)
Size
£825.00
- SKU:
- C2404-08D
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human squamous cell carcinoma cells (SCC9).
- Clone:
- 2Q995 (ASC-1)
- Format:
- Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
- Extra Details:
- The a3 subunit, also known as CD49c and VLA-3 a subunit, forms a non-covalent heterodimer with the Integrin b1 subunit (CD29). Integrin a3b1 is a receptor for laminin, fibronectin, collagen, epiligrin, and thrombospondin. Applications: Suitable for use in Inhibition of Cell Attachment, Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Inhibition of Cell Attachment: Inhibits SCC9 cell adhesion to human and EHS laminin, and laminin GD-2 peptide. Potentiates inhibition of SKOV-3 cell adhesion to EHS laminin by anti-integrin alpha 5 antibody GoH3. Does not inhibit cell adhesion to fibronectin or collagen type IV. Flow Cytometry: Stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelial cells (HUVEC). Immunohistochemistry: On acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue. Immunoprecipitation: Precipitates PAGE bands of 130kD (reduced) or 116kD and 135kD (non-reduced) from surface-biotinylated SCC9 or SKOV-3 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice