C2404-08D-BIOTIN

CD49c (Integrin alpha3, Very Late Antigen3a chain, VLA3a) (Biotin)

Size

£1,070.00

SKU:: C2404-08D-BIOTIN
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human squamous cell carcinoma cells (SCC9).
Clone:: 2Q995 (ASC-1)
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Extra Details:: The a3 subunit, also known as CD49c and VLA-3 a subunit, forms a non-covalent heterodimer with the Integrin b1 subunit (CD29). Integrin a3b1 is a receptor for laminin, fibronectin, collagen, epiligrin, and thrombospondin. Applications: Suitable for use in Inhibition of Cell Attachment, Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Inhibition of Cell Attachment: Inhibits SCC9 cell adhesion to human and EHS laminin, and laminin GD-2 peptide. Potentiates inhibition of SKOV-3 cell adhesion to EHS laminin by anti-integrin alpha 5 antibody GoH3. Does not inhibit cell adhesion to fibronectin or collagen type IV. Flow Cytometry: Stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelial cells (HUVEC). Immunohistochemistry: On acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue. Immunoprecipitation: Precipitates PAGE bands of 130kD (reduced) or 116kD and 135kD (non-reduced) from surface-biotinylated SCC9 or SKOV-3 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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