C2404-07N

CD49b (Integrin alpha2, ITGA2, alpha-2 subunit of VLA-2 Receptor, Platelet Antigen B)

Size

£787.00

SKU:: C2404-07N
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: CHO-derived rhITGA2. (aa 30 - 1132)
Clone:: 8H188
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: The Integrin alpha2 subunit, also known as CD49b and VLA-2 alpha subunit, forms a heterodimer with the Integrin Beta1 (CD29) or Beta4 (CD104) subunits and binds laminin. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: DirectELISA: 0.5-1.0ug/ml detection limit for rhITGA2 is ~ 2ng/well. Western Blot: 1-2ug/ml detection limit for rhITGA2 is ~ 5ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.