C2404-06U
CD49b (Integrin alpha2, ITGA2, alpha-2 subunit of VLA-2 Receptor, Platelet Antigen B) (PE)
Size
£448.00
- SKU:
- C2404-06U
- Application:
- Flow Cytometry
- Purity:
- Purified
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- NK cells isolated from C57BL/6 mice
- Clone:
- 5K287 (DX5)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide and a stabilizing agent. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- This antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR).2. 3 Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes.2 NK cells cultured in the presence of IL-2 progressively lose reactivity with DX5 as a consequence of cellular proliferation.1 Therefore, the antibody can be used to define functionally distinct subsets of murine NK cells. The antibody has not been demonstrated to have activating or blocking activity. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: ≤0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice