C2403-15F
CD48 (PE)
Size
£704.00
- SKU:
- C2403-15F
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human PBLs
- Clone:
- 7H129
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- Human CD48 is a GPI anchored cell surface gylcoprotein found on all lymphocytes and monocytes. CD48 is a ligand for CD2 and may be involved in enhancing the activating signal to B cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50 (10ug/ml). 80ul labels 5x10e5 cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Performance: 1. 5x10e5 cultured human Raji cells per tube were washed and incubated 45 minutes on ice with 80ul of C2403-15F at a 1:50 dilution factor (10ug/ml). 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 2.61 log10 fluorescent units when compared to a Mouse IgG2a/R-PE negative control at a similar concentration. 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD48 antibody (C2403-15C). Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
- Shipping Conditions:
- Blue Ice