C2403-15C
CD48
Size
£479.00
- SKU:
- C2403-15C
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human PBLs.
- Clone:
- 7H129/ 0.N.140
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5mg/ml gentamicin sulfate.
- Extra Details:
- Human CD48 is a GPI anchored cell surface gylcoprotein found on all lymphocytes and monocytes. CD48 is a ligand for CD2 and may be involved in enhancing the activating signal to B cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ug/ml labels 5x10e5 cultured human Raji cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured human Raji cells were washed and incubated 45 minutes on ice with 80ul of C2403-15C at a concentration of 5ug/ml. 2. Cells were washed two times and incubated with I1904-06F. 3. Washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 1.44 log10 fluorescent units when compared to I1904-83Y1 at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
- Shipping Conditions:
- Blue Ice