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C2403-14E

CD48

Size

£825.00

SKU:
C2403-14E
Purity:
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
NS0-derived recombinant protein corresponding to Gln27-Ser220 from human CD48/SLAMF2.
Clone:
8H169
Format:
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:
CD48, also known as BLAST-1, OX45, and BCM1, is a GPI-linked member of the CD2 subfamily of immunoglobulin superfamily proteins. CD48 is expressed on lymphocytes, monocytes, granulocytes, and mast cells. It functions as a co-stimulatory and adhesion molecule that binds CD2, CD229, and 2B4. CD48 also mediates bacterial phagocytosis by mast cells. Human CD48 shares approximately 50% amino acid sequence identity with mouse and rat CD48, respectively. Applications: Suitable for use in Western Blot, Flow Cytometry and Direct ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using Recombinant Human CD48/SLAMF2 under non-reducing conditions only Flow Cytometry: 0.25ug/10e6 cells using Human peripheral blood lymphocytes Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice