C2403-10A

CD48 (BCM1, BCM1 surface antigen, BLAST, BLAST1, B-lymphocyte activation marker BLAST-1, CD48 antigen, hCD48, Leukocyte antigen MEM-102, mCD48, MEM-102, SLAMF2, TCT.1)

Size

£479.00

SKU:: C2403-10A
Application:: Flow Cytometry
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human PBLs
Clone:: 5-4.8
Format:: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride. No preservative added.
Extra Details:: Human CD48 is a GPI anchored cell surface gylcoprotein found on all lymphocytes and monocytes. CD48 is a ligand for CD2 and may be involved in enhancing the activating signal to B cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured human Raji cells per tube were washed and incubated 45 min on ice with 80ul of anti-CD48 antibody at a concentration of 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.87 log10 fluorescent units when compared to a Mouse IgG2a/FITC negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.