C2402-03A-ML490
CD47 (MaxLight 490)
Size
£940.00
- SKU:
- C2402-03A-ML490
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human erythrocytes
- Clone:
- 3H1456 (BRIC126)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. CD47 is a cell surface glycoprotein that is a heavily N-glycosylated 47-52kD molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype. Applications: Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation and Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Western Blot: Recognizes CD47 under non-reducing conditions, the CD47 epitope recognized is lost on reduction Optimal dilutions to be determined by researcher. Recommended Negative Controls: I1904-84Q3: IgG2b Negative Control Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice