C2402-03A-HRP
CD47 (HRP)
Size
£940.00
- SKU:
- C2402-03A-HRP
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human erythrocytes
- Clone:
- 3H1456 (BRIC126)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- CD47 is a cell surface glycoprotein that is a heavily N-glycosylated 47-52kD molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype. Applications: Suitable for use in Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Western Blot: Recognizes CD47 under non-reducing conditions, the CD47 epitope recognized is lost on reduction Optimal dilutions to be determined by researcher. Recommended Negative Controls: I1904-84Q3: IgG2b Negative Control Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice