C2402-03A-APC

CD47 (APC)

Size

£940.00

SKU:: C2402-03A-APC
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human erythrocytes
Clone:: 3H1456 (BRIC126)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: CD47 is a cell surface glycoprotein that is a heavily N-glycosylated 47-52kD molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype. Applications: Suitable for use in Immunohistochemistry, Western Blot, Flow Cytometry and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Western Blot: Recognizes CD47 under non-reducing conditions, the CD47 epitope recognized is lost on reduction Optimal dilutions to be determined by researcher. Recommended Negative Controls: I1904-84Q3: IgG2b Negative Control Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.