C2402-02
CD47
Size
£850.00
- SKU:
- C2402-02
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Intact CD47 purified from placenta. Epitope: Ig domain Cellular Localization: Cell membrane
- Clone:
- 3F95 (B6H12.2)
- Format:
- Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
- Extra Details:
- CD47 antigen, also known as integrin associated-protein (IAP), is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. CD47 may play a role as a signal transducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Immunoprecipitation and Blocking and Inhibition Assays. Other applications not tested. Recommended Dilution: Immunofluorescence: Methanol fixed only. Immunoprecipitation (Native): 2ug/mg protein lysate. Use Protein G. Blocking Assay: Blocks binding of SIRPa. Inhibition Assay: Inhibits in assays in which CD47-integrin association is required. Optimal dilutions to be determined by the researcher. Positive Control: Tonsil Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice