C2400-70C

CD45RO (Leukocyte Common Antigen, LCA, Ly-5 T200) (FITC)

Size

£557.00

SKU:: C2400-70C
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Cultured human T cell line-CA-1
Clone:: 7H220
Format:: Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC).
Extra Details:: Human CD45 is expressed on all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane tyrosine phosphatase which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. The CD45RO isoform contains none of the alternatively spliced exons and is present on most thymocytes, a subpopulation of resting (memory) T cells within both CD4 and CD8 subsets as well as on monocytes and granulocytes. CD45RO plays an essential role in lymphocyte activation. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:40 (10ug/ml). 80ul of diluted C2400-07C labels 5x10e5 ficoll prepared human peripheral blood mononuclear cells. Immunohistochemistry: Fixed paraffin sections. Optimal dilutions to be determined by the researcher. Flow Cytometry Performance: 1. 5x10e5 ficoll prepared human peripheral blood lymphocytes were washed and pre-incubated 5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding). 2. After which they were incubated 45 minutes on ice with 80ul of C2400-70C at a 1:50 (10ug/ml) dilution. 3. Cells were washed three times, fixed and analyzed by FACS. A net 28% sub population of the cells stained positive with a mean shift of 1.05 log10 fluorescent units when compared to a Mouse IgG2a/FITC negative control at a similar concentration. Binding was blocked when pre-incubated 10 minutes with 20ul of 0.5mg/ml anti-CD45RO antibody (Catalog #C2400-70A). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

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