C2400-59C
CD45RC (Leukocyte Common Antigen HMW, LCA, Ly-5 T200) (FITC)
Size
£633.00
- SKU:
- C2400-59C
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Phytohaemagglutinin (PHA)-activated rat lymphocytes.
- Clone:
- OX-22
- Format:
- Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein Isothiocyanate (FITC).
- Extra Details:
- High molecular weight form of the leukocyte common antigen is the rat homologue of a similar antigen described in man (CD45R). The antigen is found on B cells, approximately 50% of bone marrow cells, a few percent of thymocytes, all CD8 and T cells. It splits CD4 and T cells into two populations differing in their helper activities. Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Optimal dilutions to be determined by researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice