C2400-47B-ML550

CD45RB (Leukocyte Common Antigen, LCA, Ly-5 T200) (MaxLight 550)

Size

£940.00

SKU:: C2400-47B-ML550
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Clone:: MEM55
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Extra Details:: MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP, yes Recommended Negative Controls: Mouse IgG1 Negative Control Applications: Immunohistochemistry: Frozen, Paraffin Immunoprecipitation Flow Cytometry: 1:50-1:100 Optimal working dilutions to be determined by researcher. Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Note: Applications are based on unconjugated antibody.