C2400-42J

CD45RA (CD45R, CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein)

Size

£576.00

SKU:: C2400-42J
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human thymocytes and T lymphocytes. Cellular Localization: Membrane; Single-pass type I membrane protein.
Clone:: MEM-56
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: Leukocyte common antigen is expressed exclusively on the surface of hematolymphoid cells and their progenitors, including T and B lymphocytes, granulocytes, monocytes, and macrophages. Mature erythrocytes and megakaryocytes do not express CD45. The CD45 antibody is an important component of the antibody panel used to distinguish hematolymphoid neoplasms from non-hematolymphoid neoplasms, including undifferentiated or anaplastic large cell tumors (malignant lymphoma, melanoma, and carcinoma). In a study of nasophryngeal biopsies, LCA staining was positive for all malignant melanomas and negative for all nasopharyngeal carcinomas. LCA may be used together with cytokeratin antibodies to identify the cell-lineage of anaplastic tumors, demonstrating the ability to identify lymphoma and carcinoma in up to 88% of cases in a single study. Consists of a mixture of three monoclonal antibodies: CD45, CD45RA and CD45RB. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: Crossreaction occurs with an intracellular 60kD protein of unknown identity like other anti-CD45RA Mabs. Flow Cytometry: 0.3-4ug/ml Immunohistochemistry: paraffin. No pre-treatment of tissue sections is needed. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice