C2400-28N
CD45R (B220, CD45, GP180, LCA, L-CA, Leukocyte common antigen, LY5, Receptor-type tyrosine-protein phosphatase C, T200)
Size
£479.00
- SKU:
- C2400-28N
- Application:
- Flow Cytometry
- Purity:
- Purified by size exclusion chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Periperal blood mononuclear cells
- Clone:
- 351C5
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 500mM potassium chloride, 150mM sodium chloride. No preservative added.
- Extra Details:
- Human CD45 is expressed on all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane tyrosine phosphatase which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. CD45 plays an essential role in lymphocyte activation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and pre-incubated 5 min with 20ul of 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD45R at 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC (this reagent cross reacts well with Mouse IgM). They were then washed three times, fixed and analyzed by FACS. A net 58% sub population of the cells stained positive with a mean shift of 1.80 log10 fluorescent units when compared to a Mouse IgM negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice