C2400-27D
CD45R (FITC)
Size
£639.00
- SKU:
- C2400-27D
- Application:
- Flow Cytometry
- Purity:
- ~95% pure by SDS-PAGE. Purified Immunoglobin.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Peripheral blood mononuclear cells.
- Clone:
- 7H116
- Format:
- Supplied as a liquid in 500mM potassium chloride, 150mM sodium chloride, 5% glycerol, 0.2% BSA, 0.04% sodium azide. labeled with FITC.
- Extra Details:
- Human CD45 is expressed on all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane tyrosine phosphatase which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. CD45 plays an essential role in lymphocyte activation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 ficoll prepared peripheral blood lumophocytes were washed and preincubated 5 minutes with 250ug/ml human IgG (to block non-specific binding). 2. Cells were then incubated 45 minutes on ice with 80ul of C2400-27D at a dilution of 1:50. 3. Cells washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 1.41 log10 fluorescent units when compared to a mouse IgM/FITC negative control (I1905-26E) at a similar concentration. 5. Binding was 55% blocked when cells were pre-incubated with 20ul of C2400-27B at 0.5mg/ml. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice