C2400-10R
CD45 (Receptor-type Tyrosine-protein Phosphatase C, PTPRC, Leukocyte Common Antigen, L-CA, T200) (FITC)
Size
£599.00
- SKU:
- C2400-10R
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human T lymphocytes
- Clone:
- 15j36 (F10-89-4)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- CD45 (PTPRC) is a family of high-molecular weight glycoproteins uniquely expressed on the surface of all leukocytes and their hematopoietic progenitors. The CD45 family consists of multiple members that are all products of a single gene. Structurally, CD45 is composed of one fibronectin extracellular domain, one transmembrane domain, and two cytoplasmic tyrosine phosphatase domains. It is essential for B- and T-cell activation. For both cell-types, CD45 has been shown to interact with kinases including Lyn, src family kinase Lck, and Janus-family kinases. In T cells, CD45 is required for the initiation of receptor signaling by dephosphorylating a negative regulatory tyrosine in the C-terminal tail of Lck. CD45 may also negatively regulate Lck by dephosphorylating the tyrosine in the activation loop, thereby attenuating Lck activity. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (paraffin): 1:50 Flow Cytometry: neat, 10ul labels 10e6 cells or 100ul whole blood. Optimal dilutions to be determined by the researcher. Hybridoma: NS-1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice