C2400-10B

CD45 (Protein Tyrosine Phosphatase, Receptor Type, C, B220, CD45, CD45R, GP180, LCA, L-CA, Leukocyte Common Antigen, LY5, T200)

Size

£767.00

SKU:: C2400-10B
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified T cells from human lymph nodes. Cellular Localization: Membrane; Single-pass type I membrane protein. Species sequence homology: mouse and rat.
Clone:: F10-89-4
Format:: Supplied as a liquid in PBS, 1mg/ml BSA, 10mM sodium azide.
Extra Details:: CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45. The LCA comprises of at least 5 isoforms ranging from 180-220kD which are produced as a result of alternative splicing of exons A, B or C. CBL 124 recognizes epitopes common to all of the isoforms. Antigen Distribution: Thymocytes > 95% Granulocytes > 95% Monocytes > 95% B cells (CD20+) > 95% T cells (CD3+) > 95% NK cells (CD16+) > 95% Peripheral blood lymphocytes > 95% Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:500 detected CD45 on 10ug of Jurkat lysates Immunohistochemistry: paraffin sections Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Positive Control: Jurkat cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice