C2400-05A

CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200)

Size

£364.00

SKU:: C2400-05A
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Clone:: 13/2.3
Format:: Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Extra Details:: CD45 is the common leukocyte antigen and is expressed on all cells of hematopoietic origin except erythrocytes.1-4 CD45 exists as a number of different isoforms which result from alternative RNA splicing of exons 4, 5 and 6. The CD45 molecule has been implicated as playing a key role in thymic development and in antigen-driven proliferation of T cells.4 Applications: • Flow cytometry 2, 3 • Immunoprecipitation 1, 3 • Radiometric quantitation of CD45 molecules on cell surfaces 1 • Immunohistochemistry (frozen sections) Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.