C2400-02A

CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200)

Size

£576.00

SKU:: C2400-02A
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human thymocytes and T lymphocytes.
Clone:: 6D35 (MEM-28)
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: Leukocyte common antigen (LCA) is typically at high levels on all nucleated hematopoietic cells including bone marrow cells, thymocytes, lymphocytes, monocytes, granulocytes, but not on mature erythrocytes, platelets and non-hematopoietic tissues and cells. CD45 is critically required for T and B cell antigen receptor-mediated activation. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry: 10ug/ml. Paraffin sections. No pre-treatment of tissue section is required. Western Blot: 1ug/ml; non-reducing conditions, band around 200-250kD Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.