C2399-20N
CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (PE)
Size
£730.00
- SKU:
- C2399-20N
- Application:
- Flow Cytometry
- Purity:
- Purified by Ion Exchange chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human T lymphocytes.
- Clone:
- 3H1378 (F10-89-4)
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- CD45 is a cell surface antigen. It is also known as the leukocyte common antigen (LCA). CD45 is expressed by all leukocytes, but not by non-haematopoietic tissues. Expression on lymphocytes is characteristically stronger than that observed on granulocytes. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: Neat, Use 10ul to label 10e6 cells or 100ul whole blood. Optimal dilutions to be determined by the researcher. Positive control tissue: Human tonsil. Negative Control: Mouse IgG2a Negative Control labeled with PE Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line. Source: Tissue culture supernatant Storage and Stability: Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice