C2399-08P
CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200)
Size
£550.00
- SKU:
- C2399-08P
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Mouse spleen cells.
- Clone:
- YW62.3
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
- Extra Details:
- CD45 is encoded by 3 alleles in mice, differentially expressed by various inbred strains. The Ly5 gene was originally described with the gene product LY5.1 expressed in C57bl/6 and Ly5.2 expressed in SJL strains, this was subsequently expanded to include a third allele encoding Ly5.3. Further, in 1987 a reversal of nomenclature was instigated resulting in the allele in C57bl/6 becoming Ly5b encoding Ly5.2 and the allele in SJL mice becoming Ly5a encoding Ly5.1. Further changes were made in 1992 with Ly5.1 becoming CD45.1 (SJL) and Ly5.2 becoming CD45.2 (C57bl/6). Finally, following work demonstrating homology between the CD45 antigen and a receptor linked protein tyrosine phosphatase the CD45a gene was renamed Ptprca and CD45b renamed Ptprcb. A number of different isoforms isoforms of CD45 are expressed on murine leucocytes depending on the pattern of altenative splicing of 3 exons termed A, B and C encoding regions of ~ 50 amino acids located at the N terminal region of the extracellular portion of CD45. The restricted proteins are termed CD45R with a designation depending on the expressed codon product. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Immunofluorescence. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50-1:200. Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry: Frozen sections Optimal working dilutions to be determined by researcher. Hybridoma: Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice