C2399-08F-ML650
CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (MaxLight 650)
Size
£960.00
- SKU:
- C2399-08F-ML650
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Purified B cells from mouse lymph nodes.
- Clone:
- 3H1364
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Recommended Secondary Reagents: Goat Anti Rat IgG: HRP (non-cross reacting with mouse IgG), F(ab')2 Goat anti-rat IgG Rat IgG: RPE (mouse ab Fusion Partners: Spleen cells from an immunized Wistar rat were fused with cells of the SP2/0-Ag14 mouse myeloma cell line Applications: Flow Cytometry: 1:1000 Immunohistochemistry: (frozen)- 1:500 Immunoprecipitation: 1:100 Optimal working dilutions to be determined by researcher. Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice