C2399-08F-ML490

CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (MaxLight 490)

Size

£960.00

SKU:: C2399-08F-ML490
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rat
Immunogen:: Purified B cells from mouse lymph nodes.
Clone:: 3H1364
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Extra Details:: MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Recommended Secondary Reagents: Goat Anti Rat IgG: HRP (non-cross reacting with mouse IgG), F(ab')2 Goat anti-rat IgG Rat IgG: RPE (mouse ab Fusion Partners: Spleen cells from an immunized Wistar rat were fused with cells of the SP2/0-Ag14 mouse myeloma cell line Applications: Flow Cytometry: 1:1000 Immunohistochemistry: (frozen)- 1:500 Immunoprecipitation: 1:100 Optimal working dilutions to be determined by researcher. Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Note: Applications are based on unconjugated antibody.