C2399-07G2-HRP

CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein) (HRP)

Size

£837.00

SKU:: C2399-07G2-HRP
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human T lymphocytes
Clone:: 10B1611 (F10-89-4)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Extra Details:: CD45 or Leukocyte Common Antigen (LCA/T200) family is comprised of at least four isoforms (CD45RA, CD45RB, CD45RC, and CD45RO) of membrane glycoproteins with MW of 180-220kD. It consists of a large, heavily glycosylated, amino-terminal extracellular domain of 400-550 amino acids, a single transmembrane region and a large carboxy-terminal cytoplasmic domain of 700 amino acids. CD45/LCA is expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portion of CD45 proteins has phosphatase activity and is involved in regulation of transmembrane signals, functioning as a leukocyte membrane receptor capable of transducing signals via its phosphotyrosine phosphatase domains. Applications: Suitable for use in Immunohistochemistry and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Immunohistochemistry: Frozen sections Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. EDTA, pH8.0 is recommmeded. Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Recommended Positive Control: Human Tonsil Recommended Negative Control: I1904-83P1: IgG2a Murine Negative Control Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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