C2399-03B
CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein)
Size
£607.00
- SKU:
- C2399-03B
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Rat thymocyte membrane glycoproteins
- Clone:
- OX-1
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide
- Extra Details:
- The leukocyte common antigen consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180-240kD. Antibodies recognizing a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes. CD45 isoforms play complex roles by T-cell and B-cell antigen receptor signal transduction. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100, Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry: frozen Optimal dilution determined by the researcher. Recommended Secondary Reagents: IgG Goat anti mouse IgG:FITC (rat adsorbed) IgG Goat anti mouse IgG:RPE (rat adsorbed) Immunohistology: Recommended Secondary Reagents: Goat anti mouse IgG:HRP (rat adsorbed) Hybridoma: Spleen cells from BALB/c mice were fused with cells of the NS1 mouse myeloma cell line. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice