C2399-03B-HRP
CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein) (HRP)
Size
£856.00
- SKU:
- C2399-03B-HRP
- Application:
- IHC-Fr
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Rat thymocyte membrane glycoproteins
- Clone:
- OX-1
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- The leukocyte common antigen consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180-240kD. Antibodies recognizing a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes. CD45 isoforms play complex roles by T-cell and B-cell antigen receptor signal transduction. Applications: Suitable for use in Immunohistochemistry (frozen). Other applications not tested. Recommended Dilution: Optimal dilution determined by the researcher. Recommended Secondary Reagents: IgG Goat anti mouse IgG:FITC (rat adsorbed) IgG Goat anti mouse IgG:RPE (rat adsorbed) Immunohistology: * Recommended Secondary Reagents: Goat anti mouse IgG:HRP (rat adsorbed) Hybridoma: Spleen cells from BALB/c mice were fused with cells of the NS1 mouse myeloma cell line. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice