C2399-02F

CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (PE)

Size

£704.00

SKU:: C2399-02F
Application:: Flow Cytometry
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Peripheral blood mononuclear cells
Clone:: 7H175
Format:: Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.05% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).
Extra Details:: Human CD45 is expressed on all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane tyrosine phosphatase which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. CD45 plays an essential role in lymphocyte activation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (or use 1.6ul of concentrated stock per 5x10e5 cell test). Optimal dilutions to be determined by the researcher. FACS Protocol: 1. Five x 10e5 cultured human peripheral blood mononuclear cells were washed and and preincubated ~10 minutes with 20ul human IgG at 250 ug/ml (to block nonspecific binding), after which they were incubated for 45 minutes on ice with 80ul of C2399-02F at a 1:50 dilution (10ug/ml). 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 2.4 log10 fluorescent units when compared to a IgG1 Murine Negative Control (PE) at a similar concentration. 4. Binding was blocked when pre-incubated for 10 minutes with 20ul of C2399-02C nat 0.5mg/ml Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Shipping Conditions:: Blue Ice