C2399-01B
CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200)
Size
£484.00
- SKU:
- C2399-01B
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa1029-1249 from human CD45, expressed in E. coli.
- Clone:
- 3G4
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 10% glycerol.
- Extra Details:
- CD45 belongs to a family of heavily glycosylated, high molecular weight transmembrane glycoproteins expressed on all cells of haematopoietic origin. Structurally heterogeneous, CD45 consists of a family of eight isoforms ranging from 180,000 to 220,000 MW generated by alternative splicing of three exons differentially expressed on subpopulation of lymphocytes. CD45 protein tyrosine phosphatase activity plays a central role in T-cell activation. CD45 plays an important role in signal transduction inhibition or upregulation of various immunological functions, Applications: Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 2-5ug for 1x10e6cells Western Blot: 1:1000-1:3000 Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice