C2398-93B-ML490

CD44V5 (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1) (MaxLight 490)

Size

£837.00

SKU:: C2398-93B-ML490
Purity:: Purified IgG.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Clone:: 3H1349
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Extra Details:: MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP, F(ab')2 rabbit anti mouse IgG: RPE conjugate Recommended Negative Controls: Mouse IgG1 Negative Control Applications: Western Blot Flow Cytometry: 1:50-1:100 Immunohistochemistry: (frozen)- 1:50-1:100, (paraffin)- 1:50-1:100 Optimal working dilutions to be determined by researcher. Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Immunohistochemistry: * This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium Citrate buffer pH 6.0 is recommended for this purpose. Note: Applications are based on unconjugated antibody.