C2398-15X

CD44 (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1) (BSA & Azide Free)

Size

£863.00

SKU:: C2398-15X
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Stimulated human leukocytes. Cellular Localization: Cell membrane
Clone:: 0.N.121 (156-3C11)
Format:: Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C2398-15.
Extra Details:: CD44 is a cell surface glycoprotein expressed on lymphocytes, monocytes and granulocytes. CD44 is also known as homing cell adhesion molecule (H-CAM), Phagocytic glycoprotein-1 (PgP-1), ECM-III, HUTCH-1 or Hermes-1. CD44 has been implicated in cell migration, in lymphocyte homing and in tumor metastasis. This antibody enhances IL-2 production by CD2-transfected murine hybridoma stimulated by JY cells while it has no significant effect on IL-2 production by Jurkat cells stimulated with CD3, PMA or CD2 (1). This antibody enhances proliferation of PBL stimulated with CD2 (1). Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunoprecipitation: 2ug Ab/mg protein lysate, Use Protein A; Native verified Western Blot: 1-2ug/ml for 2 hrs. at RT Immunohistochemistry: 1-2ug/ml for 30min. at RT; Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Positive Control: HUVEC or HeLa Cells. Tonsil or esophageal carcinoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice