C2398-15X-BIOTIN

CD44 (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1) (BSA & Azide Free) (Biotin)

Size

£1,119.00

SKU:: C2398-15X-BIOTIN
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Stimulated human leukocytes. Cellular Localization: Cell membrane
Clone:: 0.N.121 (156-3C11)
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Extra Details:: CD44 is a cell surface glycoprotein expressed on lymphocytes, monocytes and granulocytes. CD44 is also known as homing cell adhesion molecule (H-CAM), Phagocytic glycoprotein-1 (PgP-1), ECM-III, HUTCH-1 or Hermes-1. CD44 has been implicated in cell migration, in lymphocyte homing and in tumor metastasis. This antibody enhances IL-2 production by CD2-transfected murine hybridoma stimulated by JY cells while it has no significant effect on IL-2 production by Jurkat cells stimulated with CD3, PMA or CD2 (1). This antibody enhances proliferation of PBL stimulated with CD2 (1). Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunoprecipitation: 2ug Ab/mg protein lysate, Use Protein A; Native verified Western Blot: 1-2ug/ml for 2 hrs. at RT Immunohistochemistry: 1-2ug/ml for 30min. at RT; Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Positive Control: HUVEC or HeLa Cells. Tonsil or esophageal carcinoma Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice