C2398-06J

CD44 (CD 44 Antigen, CDw44, CSPG8, Epican, Extracellular Matrix Receptor III, ECMRIII, ECMR-III, GP90 Lymphocyte Homing Adhesion Receptor, HCAM, HCELL, Heparan Sulfate Proteoglycan, Hermes Antigen, HUTCH1, HUTCH-I, Hyaluronate Receptor, Indian Blood Group, Ly-24, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, Phagocytic Glycoprotein 1, PGP1, PGP-1, Phagocytic Glycoprotein I, PGP-I)

Size

£541.00

SKU:: C2398-06J
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: COS-7 from African Green Monkey.
Clone:: MEM-263
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumor growth and metastasis, antiapoptosis and directional motility of cancer cells. Negative on platelets and hepatocytes. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml, 60 min on vertical incubator; using: Kg-1a human acute leukemia cell lysate, Jurkat human leukemia T-cell lysate, non-reducing conditions, 6% separating gel. Flow Cytometry: 4ug/ml Immunohistochemistry (paraffin sections): 10ug/ml. Positive tissue: uterus, myometrium. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice