C2398-01N-APC

CD44 (CD 44, CDw44, ECM III, ECMRIII, Epican, Extracellular Matrix Receptor III, GP90 Lymphocyte Homing Adhesion Receptor, HCAM, HCELL, Heparan Sulfate Proteoglycan, Hermes Antigen, HUTCH1, Hyaluronate Receptor, Indian Blood Group, Inlu Related p80 Glycoprotein, lhr, Ly 24, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH1, PGP1, Phagocytic Glycoprotein 1, Phagocytic Glycoprotein I) (APC)

Size

£940.00

SKU:: C2398-01N-APC
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Clone:: Bu52
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: CD44 Iis a a 100kD glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and of the breast. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunohistochemistry (paraffin): Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Use sodium citrate buffer, pH 6, for heat mediated antigen retrieval. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice