C2398-01K-HRP

CD44 (CD 44, CDw44, ECM III, ECMRIII, Epican, Extracellular Matrix Receptor III, GP90 Lymphocyte Homing Adhesion Receptor, HCAM, HCELL, Heparan Sulfate Proteoglycan, Hermes Antigen, HUTCH1, Hyaluronate Receptor, Indian Blood Group, Inlu Related p80 Glycoprotein, lhr, Ly 24, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH1, PGP1, Phagocytic Glycoprotein 1, Phagocytic Glycoprotein I) (HRP)

Size

£940.00

SKU:: C2398-01K-HRP
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Stimulated human leukocytes.
Clone:: 10B1582 (156-3C11)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Extra Details:: CD44 is a 90kD type I transmembrane protein, otherwise known as HCAM or CD44s. Various isoforms of CD44 exist. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. Applications: Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (Frozen): 1:100 Immunohistochemistry (Paraffin): 1:100; Requires antigen retrieval using heat treatment with sodium citrate buffer, pH 6 prior to staining of paraffin sections. Western Blot: Detects a band at ~80kD in HeLa cell lysate. Optimal dilutions to be determined by the researcher. Recommended Negative Control: I1904-83P1: IgG2a Murine Negative Control Immunohistochemistry Positive Control: Human Tonstil Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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